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Antibody Product Overview
| ELK.No | ES4872 |
| Product name | Eme1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | EME1; MMS4; Crossover junction endonuclease EME1; MMS4 homolog; hMMS4 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 146956 |
| Human Swiss-Prot | Q96AY2 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Eme1 |
| Fields | >>Homologous recombination;>>Fanconi anemia pathway |
| Gene name | EME1 |
| Protein name | Crossover junction endonuclease EME1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8BJW7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from Eme1 . at AA range: 250-330 |
| Specificity | Eme1 Polyclonal Antibody detects endogenous levels of Eme1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kD |
| Background | This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009], |
| Function | cofactor:Magnesium.,function:Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.,similarity:Belongs to the EME1/MMS4 family.,subcellular location:Recruited to regions of DNA damage in S-phase cells.,subunit:May self-associate. Interacts with MUS81. Interacts with ERCC4 and FANCM., |
| Subcellular location | Nucleus, nucleolus . Recruited to regions of DNA damage in S-phase cells. |
| Expression | Epithelium,Placenta, |
Additional Images
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Western Blot analysis of COLO cells using Eme1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of EME1 Antibody. The lane on the right is blocked with the EME1 peptide.
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: AO-06-ES4872-50
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