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Antibody Product Overview
| ELK.No | ES4895 |
| Product name | SIK1 rabbit pAb |
| Reactivity | Human;Rat |
| Applications | WB;IHC |
| Other name | SIK1; SIK; SNF1LK; Serine/threonine-protein kinase SIK1; Salt-inducible kinase 1; SIK-1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 150094 |
| Human Swiss-Prot | P57059 |
| Source | Rabbit |
| Isotype | IgG |
| Target | QIK/QSK |
| Fields | >>Glucagon signaling pathway |
| Gene name | SIK1 |
| Protein name | Serine/threonine-protein kinase SIK1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q60670 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 59329 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9R1U5 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SIK. AA range:148-197 |
| Specificity | SIK1 Polyclonal Antibody detects endogenous levels of SIK1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 85kD |
| Background | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-182 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Translocates to the cytoplasm on phosphorylation where it binds binding to YWHAZ.,subunit:Binds to and is activated by YWHAZ when phosphorylated on Thr-182., |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-182 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:Transient role during the earliest stages of myocardial cell differentiation and/or primitive chamber formation and may also be important for the earliest stages of skeletal muscle growth and/or differentiation. Potential role in G2/M cell cycle regulation. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Translocates to the cytoplasm on phosp |
| Subcellular location | Cytoplasm . Nucleus . Locates to cytoplasm when inactive following cAMP-induced phosphorylation, probably by PKA (PubMed:29211348). |
| Expression | Fetal lung,Spleen,Testis, |
Additional Images
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Western Blot analysis of COLO cells using SIK1 Polyclonal Antibody
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Western blot analysis of lysates from COLO205 cells, treated with PMA 125ng/ml 30', using SIK Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES4895-100
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