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Antibody Product Overview
| ELK.No | ES4958 |
| Product name | DGK-β rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | DGKB; DAGK2; KIAA0718; Diacylglycerol kinase beta; DAG kinase beta; 90 kDa diacylglycerol kinase; Diglyceride kinase beta; DGK-beta |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1607 |
| Human Swiss-Prot | Q9Y6T7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DGK-β |
| Fields | >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Choline metabolism in cancer |
| Gene name | DGKB |
| Protein name | Diacylglycerol kinase beta |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 217480 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q6NS52 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 54248 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P49621 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DGKB. AA range:657-706 |
| Specificity | DGK-β Polyclonal Antibody detects endogenous levels of DGK-β protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 90kD |
| Background | Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Stimulated by phosphatidylserine.,function:Exhibits high phosphorylation activity for long-chain diacylglycerols.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 EF-hand domains.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers., |
| Subcellular location | Cell junction, synapse, postsynaptic cell membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cytoplasm . Translocation to the plasma membrane is induced by phorbol esters. .; [Isoform 2]: Cytoplasm . |
| Expression | [Isoform 1]: Specifically expressed in brain but also detected in uterus (PubMed:11719522). In adult brain, expressed in the amygdala, caudate nucleus, and hippocampus (PubMed:11719522). ; [Isoform 2]: More ubiquitously expressed but at lower level compared to isoform 1. |
Additional Images
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Western Blot analysis of HuvEC cells using DGK-β Polyclonal Antibody
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Western blot analysis of the lysates from HUVECcells using DGKB antibody.
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: AO-06-ES4958-100
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