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CRMP-2 (phospho Thr514) rabbit pAb
AO-06-ES5022-50
Antibody Product Overview
| ELK.No | ES5022 |
| Product name | CRMP-2 (phospho Thr514) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | DPYSL2; CRMP2; ULIP2; Dihydropyrimidinase-related protein 2; DRP-2; Collapsin response mediator protein 2; CRMP-2; N2A3; Unc-33-like phosphoprotein 2; ULIP-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 1808 |
| Human Swiss-Prot | Q16555 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CRMP-2 |
| Fields | >>Axon guidance |
| Gene name | DPYSL2 |
| Protein name | Dihydropyrimidinase-related protein 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12934 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O08553 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25416 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P47942 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DRP-2 around the phosphorylation site of Thr514. AA range:480-529 |
| Specificity | Phospho-CRMP-2 (T514) Polyclonal Antibody detects endogenous levels of CRMP-2 protein only when phosphorylated at T514. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 65kD |
| Background | This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011], |
| Function | function:Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.,PTM:3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.,similarity:Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.,subunit:Homotetramer, and heterotetramer with CRMP1, DPYSL3, DPYSL4 or DPYSL5 (By similarity). Interacts through its C-terminus with the C-terminus of CYFIP1/SRA1. Interacts with HTR4.,tissue specificity:Ubiquitous., |
| Subcellular location | Cytoplasm, cytosol . Cytoplasm, cytoskeleton . Membrane . Tightly but non-covalently associated with membranes. |
| Expression | Ubiquitous. |
Additional Images
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Western Blot analysis of 3T3 cells using Phospho-CRMP-2 (T514) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DRP-2 (Phospho-Thr514) Antibody
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Immunofluorescence analysis of HeLa cells, using DRP-2 (Phospho-Thr514) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using DRP-2 (Phospho-Thr514) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES5022-50
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