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EGFR (phospho Tyr1016) rabbit pAb
AO-06-ES5060-100
Antibody Product Overview
| ELK.No | ES5060 |
| Product name | EGFR (phospho Tyr1016) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1956 |
| Human Swiss-Prot | P00533 |
| Source | Rabbit |
| Isotype | IgG |
| Target | EGFR |
| Fields | >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Calcium signaling pathway;>>HIF-1 signaling pathway;>>FoxO signaling pathway;>>Phospholipase D signaling pathway;>>Endocytosis;>>PI3K-Akt signaling pathway;>>Focal adhesion;>>Adherens junction;>>Gap junction;>>JAK-STAT signaling pathway;>>Regulation of actin cytoskeleton;>>GnRH signaling pathway;>>Estrogen signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>Cushing syndrome;>>Epithelial cell signaling in Helicobacter pylori infection;>>Shigellosis;>>Hepatitis C;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Coronavirus disease - COVID-19;>>Pathways in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Chemical carcinogenesis - receptor activation;>>Chemical carcinogenesis - reactive oxygen species;>>Colorectal cance |
| Gene name | EGFR |
| Protein name | Epidermal growth factor receptor |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13649 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q01279 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1016. AA range:986-1035 |
| Specificity | Phospho-EGFR (Y1016) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at Y1016. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 175kD |
| Background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016], |
| Function | catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,disease:Defects in EGFR are associated with lung cancer [MIM:211980].,function:Isoform 2/truncated isoform may act as an antagonist.,function:Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin.,miscellaneous:Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.,online information:EGFR entry,PTM:Monoubiquitinated and polyubiquitinated upon EGF stimu |
| Subcellular location | Cell membrane ; Single-pass type I membrane protein . Endoplasmic reticulum membrane ; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome . Endosome membrane. Nucleus . In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER (PubMed:20674546, PubMed:17909029). Endocytosed upon activation by ligand (PubMed:2790960, PubMed:17182860, PubMed:27153536, PubMed:17909029). Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF) (PubMed:20551055). .; [Isoform 2]: Secreted. |
| Expression | Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. |
Additional Images
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Western Blot analysis of HELA cells using Phospho-EGFR (Y1016) Polyclonal Antibody diluted at 1:1000
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HeLa cells, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using EGFR (Phospho-Tyr1016) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES5060-100
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