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Antibody Product Overview
| ELK.No | ES5333 |
| Product name | LARG rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | ARHGEF12; KIAA0382; LARG; Rho guanine nucleotide exchange factor 12; Leukemia-associated RhoGEF |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 23365 |
| Human Swiss-Prot | Q9NZN5 |
| Source | Rabbit |
| Isotype | IgG |
| Target | LARG |
| Fields | >>Vascular smooth muscle contraction;>>Axon guidance;>>Platelet activation;>>C-type lectin receptor signaling pathway;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Yersinia infection;>>Tuberculosis;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer |
| Gene name | ARHGEF12 |
| Protein name | Rho guanine nucleotide exchange factor 12 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8R4H2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARHGEF12. AA range:449-498 |
| Specificity | LARG Polyclonal Antibody detects endogenous levels of LARG protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 173kD |
| Background | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014], |
| Function | disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with MLL.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain |
| Subcellular location | Cytoplasm . Membrane . Translocated to the membrane upon stimulation. . |
| Expression | Ubiquitously expressed. Isoform 2 is found in jejunum and testis. |
Additional Images
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Western Blot analysis of RAW264.7 cells using LARG Polyclonal Antibody
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Western blot analysis of lysates from RAW264.7 cells, using ARHGEF12 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using ARHGEF12 antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES5333-50
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