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DAPK2 (phospho Ser318) rabbit pAb
AO-06-ES5370-50
Antibody Product Overview
| ELK.No | ES5370 |
| Product name | DAPK2 (phospho Ser318) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | DAPK2; Death-associated protein kinase 2; DAP kinase 2; DAP-kinase-related protein 1; DRP-1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 23604 |
| Human Swiss-Prot | Q9UIK4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DAPK2 |
| Fields | >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer |
| Gene name | DAPK2 |
| Protein name | Death-associated protein kinase 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 13143 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8VDF3 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DAPK2 around the phosphorylation site of Ser318. AA range:284-333 |
| Specificity | Phospho-DAPK2 (S318) Polyclonal Antibody detects endogenous levels of DAPK2 protein only when phosphorylated at S318. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 43kD |
| Observed band (KD) | |
| Background | This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Negatively regulated by autophosphorylation on Ser-318.,function:Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.,tissue specificity:Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle., |
| Subcellular location | Cytoplasm. Cytoplasmic vesicle, autophagosome lumen. |
| Expression | Expressed in neutrophils and eosinophils (PubMed:24163421). Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using DAPK2 (Phospho-Ser318) Antibody
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Immunofluorescence analysis of COS7 cells, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using DAPK2 (Phospho-Ser318) Antibody. The picture on the right is blocked with the phospho peptide.
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: AO-06-ES5370-50
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