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Antibody Product Overview
| ELK.No | ES5409 |
| Product name | SF-1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | NR5A1; AD4BP; FTZF1; SF1; Steroidogenic factor 1; SF-1; STF-1; Adrenal 4-binding protein; Fushi tarazu factor homolog 1; Nuclear receptor subfamily 5 group A member 1; Steroid hormone receptor Ad4BP |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2516 |
| Human Swiss-Prot | Q13285 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SF-1 |
| Fields | >>Cortisol synthesis and secretion;>>Cushing syndrome |
| Gene name | NR5A1 |
| Protein name | Steroidogenic factor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 26423 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P33242 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 83826 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P50569 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NR5A1. AA range:169-218 |
| Specificity | SF-1 Polyclonal Antibody detects endogenous levels of SF-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 50kD |
| Background | The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in NR5A1 are a cause of adrenocortical insufficiency without ovarian defect [MIM:184757]. The disease is characterized by severe 'slackness,' muscular hypotonia. There is decreased sodium, increased potassium and elevated ACTH.,disease:Defects in NR5A1 are a cause of XY sex reversal with or without adrenal failure [MIM:184757]. This disease is characterized by normal female external genitalia and retention of the uterus.,function:Transcriptional activator. Seems to be essential for sexual differentiation and formation of the primary steroidogenic tissues. Binds to the Ad4 site found in the promoter region of steroidogenic P-450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the Muellerian inhibiting substance (AMH) gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1/FTZF1. The SFPQ-NO |
| Subcellular location | Nucleus . |
| Expression | High expressed in the adrenal cortex, the ovary, the testis, and the spleen (PubMed:9177385). |
Additional Images
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Western blot analysis of lysates from rat lung, using STF-1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES5409-100
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