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Antibody Product Overview
| ELK.No | ES5447 |
| Product name | Dorfin rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | RNF19A; RNF19; E3 ubiquitin-protein ligase RNF19A; Double ring-finger protein; Dorfin; RING finger protein 19A; p38 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 25897 |
| Human Swiss-Prot | Q9NV58 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Dorfin |
| Fields | |
| Gene name | RNF19A |
| Protein name | E3 ubiquitin-protein ligase RNF19A |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 30945 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P50636 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF19A. AA range:51-100 |
| Specificity | Dorfin Polyclonal Antibody detects endogenous levels of Dorfin protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 90kD |
| Background | This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013], |
| Function | function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.,pathway:Protein modification; protein ubiquitination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the RBR family. RNF19 subfamily.,similarity:Contains 1 IBR-type zinc finger.,similarity:Contains 2 RING-type zinc fingers.,subcellular location:Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.,subunit:Interacts with UBE2L3 and UBE2L6. Interac |
| Subcellular location | Membrane ; Multi-pass membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients. |
| Expression | Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system. |
Additional Images
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Western blot analysis of RNF19A Antibody. The lane on the right is blocked with the RNF19A peptide.
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Western blot analysis of the lysates from Jurkat cells using RNF19A antibody.
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: AO-06-ES5447-100
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