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TFII-I (phospho Tyr248) rabbit pAb
AO-06-ES5689-100
Antibody Product Overview
| ELK.No | ES5689 |
| Product name | TFII-I (phospho Tyr248) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | GTF2I; BAP135; WBSCR6; General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2969 |
| Human Swiss-Prot | P78347 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TFII-I |
| Fields | >>Basal transcription factors;>>cGMP-PKG signaling pathway |
| Gene name | GTF2I |
| Protein name | General transcription factor II-I |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14886 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9ESZ8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 353256 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q5U2Y1 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TFII-I around the phosphorylation site of Tyr248. AA range:214-263 |
| Specificity | Phospho-TFII-I (Y248) Polyclonal Antibody detects endogenous levels of TFII-I protein only when phosphorylated at Y248. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 115kD |
| Background | general transcription factor IIi(GTF2I) Homo sapiens This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2013], |
| Function | disease:Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.,function:Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes |
| Subcellular location | Cytoplasm . Nucleus . Colocalizes with BTK in the cytoplasm. |
| Expression | Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TFII-I (Phospho-Tyr248) Antibody
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Western blot analysis of lysates from LOVO cells, using TFII-I (Phospho-Tyr248) Antibody. The lane on the right is blocked with the phospho peptide.
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