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BLNK (phospho Tyr96) rabbit pAb
AO-06-ES5692-50
Antibody Product Overview
| ELK.No | ES5692 |
| Product name | BLNK (phospho Tyr96) rabbit pAb |
| Reactivity | Human;Mouse;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | BLNK; BASH; SLP65; B-cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; Cytoplasmic adapter protein; Src homology 2 domain-containing leukocyte protein of 65 kDa; |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 29760 |
| Human Swiss-Prot | Q8WV28 |
| Source | Rabbit |
| Isotype | IgG |
| Target | BLNK |
| Fields | >>NF-kappa B signaling pathway;>>Osteoclast differentiation;>>B cell receptor signaling pathway;>>Epstein-Barr virus infection;>>Primary immunodeficiency |
| Gene name | BLNK |
| Protein name | B-cell linker protein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17060 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9QUN3 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human BLNK around the phosphorylation site of Tyr96. AA range:62-111 |
| Specificity | Phospho-BLNK (Y96) Polyclonal Antibody detects endogenous levels of BLNK protein only when phosphorylated at Y96. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 50kD |
| Background | This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012], |
| Function | disease:Defects in BLNK are the cause of hypoglobulinemia and absent B-cells [MIM:604515]. This is a developmental blockage at the pro- to pre-B-cell transition.,disease:In 6 of 34 childhood pre-B acute lymphoblastic leukemia (ALL) samples that were tested showed a complete loss or drastic reduction of BLNK expression.,function:Functions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated ac |
| Subcellular location | Cytoplasm . Cell membrane . BCR activation results in the translocation to membrane fraction. |
| Expression | Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon. |
Additional Images
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Western Blot analysis of COLO205 cells using Phospho-BLNK (Y96) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BLNK (Phospho-Tyr96) Antibody
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Immunohistochemistry analysis of paraffin-embedded human lymph node, using BLNK (Phospho-Tyr96) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from COLO205 cells, using BLNK (Phospho-Tyr96) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES5692-50
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