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hnRNP C1/2 (phospho Ser260) rabbit pAb
AO-06-ES5754-50
Antibody Product Overview
| ELK.No | ES5754 |
| Product name | hnRNP C1/2 (phospho Ser260) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HNRNPC; HNRPC; Heterogeneous nuclear ribonucleoproteins C1/C2; hnRNP C1/C2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3183 |
| Human Swiss-Prot | P07910 |
| Source | Rabbit |
| Isotype | IgG |
| Target | hnRNP C1/C2 |
| Fields | >>Spliceosome |
| Gene name | HNRNPC |
| Protein name | Heterogeneous nuclear ribonucleoproteins C1/C2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15381 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9Z204 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | P17132 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human hnRNP C1/2 around the phosphorylation site of Ser260. AA range:231-280 |
| Specificity | Phospho-hnRNP C1/2 (S260) Polyclonal Antibody detects endogenous levels of hnRNP C1/2 protein only when phosphorylated at S260. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 41kD |
| Background | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene can act as a tetramer and is involved in the assembly of 40S hnRNP particles. Multiple transcript variants encoding at least two different isoforms have been described for this gene. [provided by RefSeq, Jul 2008], |
| Function | function:Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles. Single HNRNPC tetramers bind 230-240 nucleotides. Trimers of HNRNPC tetramers bind 700 nucleotides. May play a role in the early steps of spliceosome assembly and pre-mRNA splicing. Interacts with poly-U tracts in the 3'-UTR or 5'-UTR of mRNA and modulates the stability and the level of translation of bound mRNA molecules.,PTM:Phosphorylated on Ser-260 and Ser-299 in resting cells. Phosphorylated on Ser-253 and on 1 serine residue in the poly-Ser stretch at position 238 in response to hydrogen peroxide.,PTM:Sumoylated. Sumoylation reduces affinity for mRNA.,similarity:Belongs to the RRM HNRPC family. RALY subfamily.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Component of ribonucleosomes.,subunit:Tetramer composed of 3 copies of isoform C1 and 1 copy of isoform C2. Assembly of 3 |
| Subcellular location | Nucleus. Component of ribonucleosomes. |
| Expression | Blood vessels- blood vessel,Bone marrow,Brain,Cajal |
Additional Images
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Western Blot analysis of 293 cells using Phospho-hnRNP C1/2 (S260) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using hnRNP C1/2 (Phospho-Ser260) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with H2O2 100uM 15', using hnRNP C1/2 (Phospho-Ser260) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES5754-50
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