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XIAP (phospho Ser87) rabbit pAb
AO-06-ES5786-100
Antibody Product Overview
| ELK.No | ES5786 |
| Product name | XIAP (phospho Ser87) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | XIAP; API3; BIRC4; IAP3; E3 ubiquitin-protein ligase XIAP; Baculoviral IAP repeat-containing protein 4; IAP-like protein; ILP; hILP; Inhibitor of apoptosis protein 3; IAP-3; hIAP-3; hIAP3; X-linked inhibitor of apoptosis protein; X-linked I |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 331 |
| Human Swiss-Prot | P98170 |
| Source | Rabbit |
| Isotype | IgG |
| Target | XIAP |
| Fields | >>Platinum drug resistance;>>NF-kappa B signaling pathway;>>Ubiquitin mediated proteolysis;>>Apoptosis;>>Apoptosis - multiple species;>>Necroptosis;>>Focal adhesion;>>NOD-like receptor signaling pathway;>>Toxoplasmosis;>>Human T-cell leukemia virus 1 infection;>>Pathways in cancer;>>Chemical carcinogenesis - receptor activation;>>Small cell lung cancer |
| Gene name | XIAP |
| Protein name | E3 ubiquitin-protein ligase XIAP |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11798 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q60989 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 63879 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9R0I6 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human XIAP around the phosphorylation site of Ser87. AA range:53-102 |
| Specificity | Phospho-XIAP (S87) Polyclonal Antibody detects endogenous levels of XIAP protein only when phosphorylated at S87. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 57kD |
| Background | This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011], |
| Function | disease:Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.,domain:The first BIR domain is involved in interaction with MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.,function:Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, lead |
| Subcellular location | Cytoplasm. Nucleus. TLE3 promotes its nuclear localization. |
| Expression | Expressed in colonic crypts (at protein level) (PubMed:30389919). Ubiquitous, except peripheral blood leukocytes (PubMed:8654366). |
Additional Images
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Western Blot analysis of HepG2 cells using Phospho-XIAP (S87) Polyclonal Antibody diluted at 1:500
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using XIAP (Phospho-Ser87) Antibody
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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using XIAP (Phospho-Ser87) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HepG2 cells treated with Anisomycin 25ug/ml 30', using XIAP (Phospho-Ser87) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES5786-100
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