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Antibody Product Overview
| ELK.No | ES5794 |
| Product name | HSP10 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HSPE1; 10 kDa heat shock protein; mitochondrial; Hsp10; 10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3336 |
| Human Swiss-Prot | P61604 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HSP10 |
| Fields | |
| Gene name | HSPE1 |
| Protein name | 10 kDa heat shock protein mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15528 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q64433 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | P26772 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP10. AA range:51-100 |
| Specificity | HSP10 Polyclonal Antibody detects endogenous levels of HSP10 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 10kD |
| Background | This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011], |
| Function | function:Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.,induction:By stress.,mass spectrometry: PubMed:7912672,similarity:Belongs to the groES chaperonin family.,subunit:Homohexamer., |
| Subcellular location | Mitochondrion matrix. |
| Expression | B-cell lymphoma,Uterus, |
Additional Images
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Western Blot analysis of 3T3 cells using HSP10 Polyclonal Antibody
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Immunofluorescence analysis of NIH/3T3 cells, using HSP10 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using HSP10 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from NIH/3T3 cells, using HSP10 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES5794-100
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