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Antibody Product Overview
| ELK.No | ES5805 |
| Product name | SR-5A rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IF;ELISA |
| Other name | HTR5A; 5-hydroxytryptamine receptor 5A; 5-HT-5; 5-HT-5A; 5-HT5A; Serotonin receptor 5A |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3361 |
| Human Swiss-Prot | P47898 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SR-5A |
| Fields | >>Calcium signaling pathway;>>Neuroactive ligand-receptor interaction;>>Serotonergic synapse |
| Gene name | HTR5A |
| Protein name | 5-hydroxytryptamine receptor 5A |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P30966 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HTR5A. AA range:15-64 |
| Specificity | SR-5A Polyclonal Antibody detects endogenous levels of SR-5A protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 36kD |
| Background | The neurotransmitter serotonin (5-hydroxytryptamine, 5-HT) has been implicated in a wide range of psychiatric conditions and also has vasoconstrictive and vasodilatory effects. The gene described in this record is a member of 5-hydroxytryptamine (serotonin) receptor family and encodes a multi-pass membrane protein that functions as a receptor for 5-hydroxytryptamine and couples to G-proteins. This protein has been shown to function in part through the regulation of intracellular Ca2+ mobilization. [provided by RefSeq, Jul 2008], |
| Function | function:This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins.,similarity:Belongs to the G-protein coupled receptor 1 family., |
| Subcellular location | Cell membrane; Multi-pass membrane protein. |
| Expression | Brain,Cerebellum, |
Additional Images
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Immunofluorescence analysis of LOVO cells, using HTR5A Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using HTR5A Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using HTR5A antibody.
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: AO-06-ES5805-50
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