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Antibody Product Overview
| ELK.No | ES5811 |
| Product name | CD50 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IF;ELISA |
| Other name | ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3385 |
| Human Swiss-Prot | P32942 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CD50/ICAM-3 |
| Fields | >>Cell adhesion molecules |
| Gene name | ICAM3 |
| Protein name | Intercellular adhesion molecule 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD50/ICAM-3. AA range:484-533 |
| Specificity | CD50 Polyclonal Antibody detects endogenous levels of CD50 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 42-60kD |
| Background | The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is constitutively and abundantly expressed by all leucocytes and may be the most important ligand for LFA-1 in the initiation of the immune response. It functions not only as an adhesion molecule, but also as a potent signalling molecule. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2016], |
| Function | function:ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM3 is also a ligand for integrin alpha-D/beta-2.,online information:ICAM-3,PTM:N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.,PTM:Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.,similarity:Belongs to the immunoglobulin superfamily. ICAM family.,similarity:Contains 5 Ig-like C2-type (immunoglobulin-like) domains., |
| Subcellular location | Membrane; Single-pass type I membrane protein. |
| Expression | Leukocytes. |
Additional Images
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Western Blot analysis of NIH-3T3 cells using CD50 Polyclonal Antibody
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Immunofluorescence analysis of A549 cells, using CD50/ICAM-3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from NIH/3T3 cells, treated with TNF-a 20ng/ml 5', using CD50/ICAM-3 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES5811-100
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