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IRS-1 (phospho Ser323) rabbit pAb
AO-06-ES5928-50
Antibody Product Overview
| ELK.No | ES5928 |
| Product name | IRS-1 (phospho Ser323) rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | IRS1; Insulin receptor substrate 1; IRS-1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3667 |
| Human Swiss-Prot | P35568 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IRS-1 |
| Fields | >>cGMP-PKG signaling pathway;>>FoxO signaling pathway;>>Autophagy - animal;>>mTOR signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Neurotrophin signaling pathway;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Regulation of lipolysis in adipocytes;>>Type II diabetes mellitus;>>Insulin resistance;>>Non-alcoholic fatty liver disease;>>Growth hormone synthesis, secretion and action;>>Aldosterone-regulated sodium reabsorption;>>Alzheimer disease;>>MicroRNAs in cancer;>>Diabetic cardiomyopathy |
| Gene name | IRS1 |
| Protein name | Insulin receptor substrate 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16367 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P35569 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25467 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P35570 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser323. AA range:289-338 |
| Specificity | Phospho-IRS-1 (S323) Polyclonal Antibody detects endogenous levels of IRS-1 protein only when phosphorylated at S323. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 170kD |
| Background | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009], |
| Function | disease:Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].,function:May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.,polymorphism:The Arg-971 polymorphism impairs the ability of insulin to stimulate glucose transport, glucose transporter translocation, and glycogen synthesis by affecting the PI3K/AKT1/GSK3 signaling pathway. The polymorphism at Arg-971 may contribute to the in vivo insulin resistance observed in carriers of this variant. Arg-971 could contribute to the risk for atherosclerotic cardiovascular diseases associated with non-insulin-dependen |
| Subcellular location | nucleus,cytoplasm,cytosol,plasma membrane,insulin receptor complex,caveola,intracellular membrane-bounded organelle, |
| Expression | Epithelium,Eye,Skeletal muscle, |
Additional Images
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Western blot analysis of 293T using p-IRS-1 (S323) antibody. Antibody was diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRS-1 (Phospho-Ser323) Antibody
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Immunofluorescence analysis of HeLa cells, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser323) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES5928-50
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