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Antibody Product Overview
| ELK.No | ES5955 |
| Product name | A-Raf rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;ELISA |
| Other name | ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 369 |
| Human Swiss-Prot | P10398 |
| Source | Rabbit |
| Isotype | IgG |
| Target | A-Raf |
| Fields | >>EGFR tyrosine kinase inhibitor resistance;>>Endocrine resistance;>>MAPK signaling pathway;>>ErbB signaling pathway;>>FoxO signaling pathway;>>Vascular smooth muscle contraction;>>Natural killer cell mediated cytotoxicity;>>Long-term potentiation;>>Serotonergic synapse;>>Long-term depression;>>Regulation of actin cytoskeleton;>>Insulin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Parathyroid hormone synthesis, secretion and action;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Alcoholism;>>Hepatitis C;>>Hepatitis B;>>Pathways in cancer;>>Proteoglycans in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Colorectal cancer;>>Renal cell carcinoma;>>Pancreatic cancer;>>Endometrial cancer;>>Glioma;>>Prostate cancer;>>Melanoma;>>Bladder cancer;>>Chronic myeloid leukemia;>>Acute myeloid leukemia;>>Non-small cell lung cancer;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer |
| Gene name | ARAF |
| Protein name | Serine/threonine-protein kinase A-Raf |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11836 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P04627 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 64363 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P14056 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human A-RAF. AA range:241-290 |
| Specificity | A-Raf Polyclonal Antibody detects endogenous levels of A-Raf protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 68kD |
| Background | This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Binds 2 zinc ions per subunit.,function:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RBD (Ras-binding) domain.,subunit:Interacts with TH1L/NELFD.,tissue specificity:Predominantly in urogenital tissues., |
| Subcellular location | intracellular,mitochondrion,cytosol, |
| Expression | Predominantly in urogenital tissues. |
Additional Images
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Western Blot analysis of 293T cells using A-Raf Polyclonal Antibody diluted at 1:500
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Western blot analysis of the lysates from HeLa cells using A-RAF antibody.
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: AO-06-ES5955-100
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