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Antibody Product Overview
| ELK.No | ES6118 |
| Product name | Rho H rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RHOH; ARHH; TTF; Rho-related GTP-binding protein RhoH; GTP-binding protein TTF; Translocation three four protein |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 399 |
| Human Swiss-Prot | Q15669 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rho H |
| Fields | >>Leukocyte transendothelial migration;>>Salmonella infection |
| Gene name | RhoH |
| Protein name | Rho-related GTP-binding protein RhoH |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 74734 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D3G9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RhoH. AA range:141-190 |
| Specificity | Rho H Polyclonal Antibody detects endogenous levels of Rho H protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 21kD |
| Background | The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013], |
| Function | disease:A chromosomal aberration involving RHOH is found in a non-Hodgkin lymphoma cell line. Translocation t(3;4)(q27;p11) with BCL6.,similarity:Belongs to the small GTPase superfamily. Rho family.,tissue specificity:Transcribed only in hemopoietic cells., |
| Subcellular location | Cytoplasm . Cell membrane ; Lipid-anchor ; Cytoplasmic side . Colocalizes together with ZAP70 in the immunological synapse. . |
| Expression | Expressed only in hematopoietic cells. Present at very high levels in the thymus, less abundant in the spleen, and least abundant in the bone marrow. Expressed at a higher level in the TH1 subtype of T-helper cells than in the TH2 subpopulation. Expressed in neutrophils under inflammatory conditions, such as cystic fibrosis, ulcerative colitis and appendicitis. |
Additional Images
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Immunofluorescence analysis of A549 cells, using RhoH Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HT-29 cells, using RhoH Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from RAW264.7cells using RhoH antibody.
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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200
: AO-06-ES6118-50
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