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HSL (phospho Ser855) rabbit pAb
AO-06-ES6120-100
Antibody Product Overview
| ELK.No | ES6120 |
| Product name | HSL (phospho Ser855) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | LIPE; Hormone-sensitive lipase; HSL |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3991 |
| Human Swiss-Prot | Q05469 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HSL |
| Fields | >>cAMP signaling pathway;>>AMPK signaling pathway;>>Apelin signaling pathway;>>Thermogenesis;>>Insulin signaling pathway;>>Regulation of lipolysis in adipocytes;>>Aldosterone synthesis and secretion |
| Gene name | LIPE |
| Protein name | Hormone-sensitive lipase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16890 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P54310 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25330 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P15304 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554. AA range:520-569 |
| Specificity | Phospho-HSL (S855) Polyclonal Antibody detects endogenous levels of HSL protein only when phosphorylated at S855. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 81kD |
| Background | The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:Diacylglycerol + H(2)O = monoacylglycerol + a carboxylate.,catalytic activity:Monoacylglycerol + H(2)O = glycerol + a carboxylate.,catalytic activity:Triacylglycerol + H(2)O = diacylglycerol + a carboxylate.,enzyme regulation:Rapidly activated by cAMP-dependent phosphorylation under the influence of catecholamines. Dephosphorylation and inactivation are controlled by insulin.,function:In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.,pathway:Glycerolipid metabolism; triacylglycerol degradation.,similarity:Belongs to the 'GDXG' lipolytic enzyme family.,subcellular location:Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.,subunit:Interacts wi |
| Subcellular location | Cell membrane . Membrane, caveola . Cytoplasm, cytosol . Lipid droplet . Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation (PubMed:17026959). Phosphorylation by AMPK reduces its translocation towards the lipid droplets (By similarity). . |
| Expression | Testis. |
Additional Images
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Western blot analysis of L929 using p-HSL (S855) antibody. Antibody was diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSL (Phospho-Ser855/554) Antibody
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ng/ml 24h, using HSL (Phospho-Ser855/554) Antibody. The lane on the right is blocked with the phospho peptide.
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