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Mcl-1 (phospho Ser159) rabbit pAb
AO-06-ES6205-100
Antibody Product Overview
| ELK.No | ES6205 |
| Product name | Mcl-1 (phospho Ser159) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | MCL1; BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 4170 |
| Human Swiss-Prot | Q07820 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Mcl-1 |
| Fields | >>PI3K-Akt signaling pathway;>>Apoptosis;>>JAK-STAT signaling pathway;>>MicroRNAs in cancer |
| Gene name | MCL1 |
| Protein name | Induced myeloid leukemia cell differentiation protein Mcl-1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17210 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97287 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MCL1 around the phosphorylation site of Ser159. AA range:125-174 |
| Specificity | Phospho-Mcl-1 (S159) Polyclonal Antibody detects endogenous levels of Mcl-1 protein only when phosphorylated at S159. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | About 40kd in human,39kd in mouse and rat |
| Background | This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010], |
| Function | function:Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis while isoform 2 promotes it.,induction:Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML-1. Rapidly up-regulated by CSF2 in ML-1 cells. Up-regulated by heat-shock induced differentiation. Expression increases early during retinoic acid-induced differentiation.,PTM:Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.,PTM:Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.,PTM:Rapidly degraded in the abs |
| Subcellular location | Membrane ; Single-pass membrane protein . Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Cytoplasmic, associated with mitochondria. |
| Expression | Ewing sarcoma,Mammary gland,Myeloid leukemia cell,Neuroblastoma,Placenta,Th |
Additional Images
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Western blot analysis of K562 using p-Mcl-1 (S159) antibody.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MCL1 (Phospho-Ser159) Antibody
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Immunofluorescence analysis of A549 cells, using MCL1 (Phospho-Ser159) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES6205-100
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