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Moesin/Ezrin/Radixin (phospho Thr558) rabbit pAb
AO-06-ES6283-50
Antibody Product Overview
| ELK.No | ES6283 |
| Product name | Moesin/Ezrin/Radixin (phospho Thr558) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4478/5962 |
| Human Swiss-Prot | P26038/P35241/P15311 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Moesin/Ezrin/Radixin |
| Fields | >>Tight junction;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Measles;>>Proteoglycans in cancer |
| Gene name | MSN |
| Protein name | Moesin |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17698/19684/22350 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 81521/54319 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O35763/P31977 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Moesin/Ezrin/Radixin around the phosphorylation site of Thr558. AA range:524-573 |
| Specificity | Phospho-Moesin/Ezrin/Radixin (T558) Polyclonal Antibody detects endogenous levels of Moesin/Ezrin/Radixin protein only when phosphorylated at T558. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 67kD |
| Background | Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq, Jul 2008], |
| Function | function:Probably involved in connections of major cytoskeletal structures to the plasma membrane.,PTM:Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.,similarity:Contains 1 FERM domain.,subcellular location:Phosphorylated form is enriched in microvilli-like structures at apical membrane.,subunit:In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). Binds SLC9A3R1.,tissue specificity:In all tissues and cultured cells studied., |
| Subcellular location | Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Apical cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, microvillus . Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment (By similarity). Localizes at the uropods of T lymphoblasts. . |
| Expression | In all tissues and cultured cells studied. |
Additional Images
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Immunofluorescence analysis of A549 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human pancreas, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from NIH/3T3 cells, using Moesin/Ezrin/Radixin (Phospho-Thr558) Antibody. The lane on the right is blocked with the phospho peptide.
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