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eNOS (phospho Thr494) rabbit pAb
AO-06-ES6399-50
Antibody Product Overview
| ELK.No | ES6399 |
| Product name | eNOS (phospho Thr494) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4846 |
| Human Swiss-Prot | P29474 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NOS3/eNOS |
| Fields | >>Arginine biosynthesis;>>Arginine and proline metabolism;>>Metabolic pathways;>>Calcium signaling pathway;>>cGMP-PKG signaling pathway;>>HIF-1 signaling pathway;>>Sphingolipid signaling pathway;>>PI3K-Akt signaling pathway;>>VEGF signaling pathway;>>Apelin signaling pathway;>>Platelet activation;>>Estrogen signaling pathway;>>Oxytocin signaling pathway;>>Relaxin signaling pathway;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis |
| Gene name | NOS3 |
| Protein name | Nitric oxide synthase endothelial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18127 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P70313 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24600 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q62600 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Thr494. AA range:462-511 |
| Specificity | Phospho-NOS3 (T494) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at T494. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 130-140kD |
| Background | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009], |
| Function | catalytic activity:L-arginine + n NADPH + n H(+) + m O(2) = citrulline + nitric oxide + n NADP(+).,cofactor:Binds 1 FAD.,cofactor:Binds 1 FMN.,cofactor:Heme group.,cofactor:Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.,enzyme regulation:Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.,function:Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.,online information:Nitric oxide synthase entry,polymorphism:Variation in NOS3 seem to be associated with susceptibility to coronary spasm.,similarity:Belongs to the NOS family.,similarity:Contains 1 FAD-binding FR-type domain.,similarity:Contains 1 flavodoxin-like |
| Subcellular location | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Expression | Platelets, placenta, liver and kidney. |
Additional Images
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Western Blot analysis of 293 cells using Phospho-NOS3 (T494) Polyclonal Antibody diluted at 1:1000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using eNOS (Phospho-Thr494) Antibody
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Western blot analysis of lysates from Jurkat cells, using eNOS (Phospho-Thr494) Antibody. The lane on the right is blocked with the phospho peptide.
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