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Antibody Product Overview
| ELK.No | ES6419 |
| Product name | NSF rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | NSF; Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4905 |
| Human Swiss-Prot | P46459 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NSF |
| Fields | >>Synaptic vesicle cycle;>>GABAergic synapse;>>Vasopressin-regulated water reabsorption |
| Gene name | NSF |
| Protein name | Vesicle-fusing ATPase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18195 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P46460 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 60355 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9QUL6 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from NSF . at AA range: 110-190 |
| Specificity | NSF Polyclonal Antibody detects endogenous levels of NSF protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 75kD |
| Background | catalytic activity:ATP + H(2)O = ADP + phosphate.,cofactor:Binds 1 magnesium ion per subunit.,function:Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.,similarity:Belongs to the AAA ATPase family.,subunit:Homohexamer. Interacts with GABARAP and GABARAPL2., |
| Function | catalytic activity:ATP + H(2)O = ADP + phosphate.,cofactor:Binds 1 magnesium ion per subunit.,function:Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.,similarity:Belongs to the AAA ATPase family.,subunit:Homohexamer. Interacts with GABARAP and GABARAPL2., |
| Subcellular location | Cytoplasm. |
| Expression | Brain,Kidney,Lung, |
Additional Images
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Western Blot analysis of CoLo cells using NSF Polyclonal Antibody
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: AO-06-ES6419-50
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