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PC-PLD1 (phospho Ser561) rabbit pAb
AO-06-ES6612-100
Antibody Product Overview
| ELK.No | ES6612 |
| Product name | PC-PLD1 (phospho Ser561) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | PLD1; Phospholipase D1; PLD 1; hPLD1; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5337 |
| Human Swiss-Prot | Q13393 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PC-PLD1 |
| Fields | >>Glycerophospholipid metabolism;>>Ether lipid metabolism;>>Metabolic pathways;>>Ras signaling pathway;>>cAMP signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Endocytosis;>>Fc gamma R-mediated phagocytosis;>>Glutamatergic synapse;>>GnRH signaling pathway;>>Parathyroid hormone synthesis, secretion and action;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Pancreatic cancer;>>Choline metabolism in cancer |
| Gene name | PLD1 |
| Protein name | Phospholipase D1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9Z280 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25096 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P70496 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLD1 around the phosphorylation site of Ser561. AA range:527-576 |
| Specificity | Phospho-PC-PLD1 (S561) Polyclonal Antibody detects endogenous levels of PC-PLD1 protein only when phosphorylated at S561. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 124kD |
| Observed band (KD) | |
| Background | This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011], |
| Function | catalytic activity:A phosphatidylcholine + H(2)O = choline + a phosphatidate.,enzyme regulation:Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42. Inhibited by oleate.,function:Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic.,online information:Phospholipase D entry,similarity:Belongs to the phospholipase D family.,similarity:Contains 1 PH domain.,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 PLD phosphodiesterase domains.,subunit:Interacts with PIP5K1A.,tissue specificity:Expressed abundant |
| Subcellular location | Cytoplasm, perinuclear region . Endoplasmic reticulum membrane ; Lipid-anchor ; Cytoplasmic side . Golgi apparatus membrane ; Lipid-anchor ; Cytoplasmic side . Late endosome membrane ; Lipid-anchor ; Cytoplasmic side . |
| Expression | Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLD1 (Phospho-Ser561) Antibody
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Immunofluorescence analysis of HepG2 cells, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using PLD1 (Phospho-Ser561) Antibody. The picture on the right is blocked with the phospho peptide.
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