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Antibody Product Overview
| ELK.No | ES6643 |
| Product name | DNA pol δ cat rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | POLD1; POLD; DNA polymerase delta catalytic subunit; DNA polymerase subunit delta p125 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5424 |
| Human Swiss-Prot | P28340 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DNA pol δ cat |
| Fields | >>DNA replication;>>Base excision repair;>>Nucleotide excision repair;>>Mismatch repair;>>Homologous recombination |
| Gene name | POLD1 |
| Protein name | DNA polymerase delta catalytic subunit |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18971 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P52431 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 59294 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O54747 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human POLD1. AA range:1051-1100 |
| Specificity | DNA pol δ cat Polyclonal Antibody detects endogenous levels of DNA pol δ cat protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 110 124kD |
| Background | This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012], |
| Function | catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Heterotetramer composed of subunits of 125 kDa, 50 kDa, 66 kDa and 12 kDa. The 125 kDa subunit contains the polymerase active site and most likely the active site for |
| Subcellular location | Nucleus . Colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). After UV irradiation, recruited to DNA damage sites within 2 hours, independently on the cell cycle phase, nor on PCNA ubiquitination. This recruitment requires POLD3, PCNA and RFC1-replication factor C complex (PubMed:20227374, PubMed:22801543). . |
| Expression | Widely expressed, with high levels of expression in heart and lung. |
Additional Images
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Western blot analysis of lysates from K562 cells, using POLD1 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES6643-50
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