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Antibody Product Overview
| ELK.No | ES6707 |
| Product name | NIPP1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | PPP1R8; ARD1; NIPP1; Nuclear inhibitor of protein phosphatase 1; NIPP-1; Protein phosphatase 1 regulatory inhibitor subunit 8 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5511 |
| Human Swiss-Prot | Q12972 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NIPP1 |
| Fields | |
| Gene name | PPP1R8 |
| Protein name | Nuclear inhibitor of protein phosphatase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 100336 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8R3G1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PPP1R8. AA range:196-245 |
| Specificity | NIPP1 Polyclonal Antibody detects endogenous levels of NIPP1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul 2008], |
| Function | cofactor:Magnesium. Endoribonuclease function is magnesium-dependent.,domain:Has a basic N- and C-terminal and an acidic central domain.,function:Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.,function:Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.,miscellaneous:A synthetic peptide, NIPP-1(330-351), is able to inhibit PP-1. Phosphorylation of Tyr-335 reduces PP-1 inhibition, whereas phosphorylation |
| Subcellular location | Nucleus. Nucleus speckle. Primarily, but not exclusively, nuclear.; [Isoform Gamma]: Cytoplasm. Found mainly in the cytoplasm. |
| Expression | Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. |
Additional Images
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Western blot analysis of lysates from Jurkat cells, using PPP1R8 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using PPP1R8 antibody.
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: AO-06-ES6707-100
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