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PKC ζ (phospho Thr560) rabbit pAb
AO-06-ES6800-50
Antibody Product Overview
| ELK.No | ES6800 |
| Product name | PKC ζ (phospho Thr560) rabbit pAb |
| Reactivity | Human;Mouse;Rat;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5590 |
| Human Swiss-Prot | Q05513 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PKC ζ |
| Fields | >>Rap1 signaling pathway;>>Chemokine signaling pathway;>>Sphingolipid signaling pathway;>>Endocytosis;>>Axon guidance;>>Hippo signaling pathway;>>Tight junction;>>Platelet activation;>>Insulin signaling pathway;>>Relaxin signaling pathway;>>Type II diabetes mellitus;>>Insulin resistance;>>AGE-RAGE signaling pathway in diabetic complications;>>Human papillomavirus infection;>>Diabetic cardiomyopathy;>>Fluid shear stress and atherosclerosis |
| Gene name | PRKCZ |
| Protein name | Protein kinase C zeta type |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18762 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q02956 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25522 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P09217 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC zeta around the phosphorylation site of Thr560. AA range:526-575 |
| Specificity | Phospho-PKC ζ (T560) Polyclonal Antibody detects endogenous levels of PKC ζ protein only when phosphorylated at T560. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,domain:The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.,enzyme regulation:Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator. Two specific sites, Thr-410 (activation loop of the kinase domain) and Thr-560 (turn motif), need to be phosphorylated for its full activation.,function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.,function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to |
| Subcellular location | Cytoplasm . Endosome . Cell junction . Membrane ; Peripheral membrane protein . In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes (PubMed:9566925). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction (PubMed:7597083). Colocalizes with VAMP2 and WDFY2 in intracellular vesicles (PubMed:17313651). Transiently translocates to the membrane of CA1 hippocampal cells in response to the induction of long term potentiation (By similarity). .; [Isoform 2]: Cytoplasm . |
| Expression | Expressed in brain, and to a lesser extent in lung, kidney and testis. |
Additional Images
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Western Blot analysis of HELA 293T cells using Phospho-PKC ζ (T560) Polyclonal Antibody diluted at 1:2000
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody
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Immunohistochemistry analysis of paraffin-embedded human brain, using PKC zeta (Phospho-Thr560) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKC zeta (Phospho-Thr560) Antibody. The lane on the right is blocked with the phospho peptide.
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