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Antibody Product Overview
| ELK.No | ES6871 |
| Product name | MRP-L47 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC |
| Other name | MRPL47; NCM1; CGI-204; 39S ribosomal protein L47; mitochondrial; L47mt; MRP-L47; Nasopharyngeal carcinoma metastasis-related protein 1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 57129 |
| Human Swiss-Prot | Q9HD33 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-L47 |
| Fields | |
| Gene name | MRPL47 |
| Protein name | 39S ribosomal protein L47 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8K2Y7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL47. AA range:191-240 |
| Specificity | MRP-L47 Polyclonal Antibody detects endogenous levels of MRP-L47 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 30kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identifie |
| Function | sequence caution:Chimeric cDNA. Seems to include a portion of the orthologous murine sequence.,similarity:Belongs to the ribosomal protein L47em family., |
| Subcellular location | Mitochondrion . |
| Expression | Lymph, |
Additional Images
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Western blot analysis of lysates from RAW264.7 and K562 cells, using MRPL47 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using MRPL47 antibody.
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES6871-100
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