Français
English
Antibody Product Overview
| ELK.No | ES7210 |
| Product name | MRP-L32 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | MRPL32; HSPC283; 39S ribosomal protein L32; mitochondrial; L32mt; MRP-L32 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 64983 |
| Human Swiss-Prot | Q9BYC8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-L32 |
| Fields | >>Ribosome |
| Gene name | MRPL32 |
| Protein name | 39S ribosomal protein L32 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9DCI9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL32. AA range:101-150 |
| Specificity | MRP-L32 Polyclonal Antibody detects endogenous levels of MRP-L32 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 21kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008], |
| Function | similarity:Belongs to the ribosomal protein L32P family., |
| Subcellular location | Mitochondrion . |
| Expression | Lymph,Trachea,Umbilical cord blood, |
Additional Images
No image
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
No image
Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from MCF-7, HepG2, and Jurkat cells, using MRPL32 Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Western blot analysis of the lysates from HeLa cells using MRPL32 antibody.
: AO-06-ES7210-100
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package