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p47-phox (phospho Ser359) rabbit pAb
AO-06-ES7222-50
Antibody Product Overview
| ELK.No | ES7222 |
| Product name | p47-phox (phospho Ser359) rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | NCF1; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 653361 |
| Human Swiss-Prot | P14598 |
| Source | Rabbit |
| Isotype | IgG |
| Target | p47-phox |
| Fields | >>Chemokine signaling pathway;>>Phagosome;>>Osteoclast differentiation;>>Neutrophil extracellular trap formation;>>Fc gamma R-mediated phagocytosis;>>Leukocyte transendothelial migration;>>Prion disease;>>Leishmaniasis;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy;>>Lipid and atherosclerosis;>>Fluid shear stress and atherosclerosis |
| Gene name | NCF1 |
| Protein name | Neutrophil cytosol factor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q09014 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser359. AA range:331-380 |
| Specificity | Phospho-p47-phox (S359) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S359. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.,function:NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).,online information:NCF1 deficiency database,similarity:Contains 1 PX (phox homology) domain.,similarity:Contains 2 SH3 domains.,subunit:Interacts with NOXA1., |
| Subcellular location | Cytoplasm, cytosol . Membrane ; Peripheral membrane protein ; Cytoplasmic side . |
| Expression | Detected in peripheral blood monocytes and neutrophils (at protein level). |
Additional Images
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Western Blot analysis of HeLa nocodazole 1ug/ml 18h cells using Phospho-p47-phox (S359) Polyclonal Antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of HeLa cells, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using p47 phox (Phospho-Ser359) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES7222-50
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