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Antibody Product Overview
| ELK.No | ES7244 |
| Product name | POLR3D rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | POLR3D; BN51; BN51T; DNA-directed RNA polymerase III subunit RPC4; RNA polymerase III subunit C4; DNA-directed RNA polymerase III subunit D; Protein BN51; RNA polymerase III 47 kDa subunit; RPC53 homolog |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 661 |
| Human Swiss-Prot | P05423 |
| Source | Rabbit |
| Isotype | IgG |
| Target | POLR3D |
| Fields | >>RNA polymerase;>>Cytosolic DNA-sensing pathway |
| Gene name | POLR3D |
| Protein name | DNA-directed RNA polymerase III subunit RPC4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67065 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q91WD1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RPC4. AA range:281-330 |
| Specificity | POLR3D Polyclonal Antibody detects endogenous levels of POLR3D protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 38kD |
| Background | This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures. [provided by RefSeq, Jul 2008], |
| Function | function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs.,similarity:Belongs to the eukaryotic RPC4/POLR3D RNA polymerase subunit family.,subunit:Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). Interacts with POLR3E/RPC5., |
| Subcellular location | Nucleus. |
| Expression | Epithelium,Lung,Lymph, |
Additional Images
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Western blot analysis of lysates from MCF-7 cells, using RPC4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using RPC4 antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES7244-100
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