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Antibody Product Overview
| ELK.No | ES7583 |
| Product name | CHSY2 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CHPF; CSS2; Chondroitin sulfate synthase 2; Chondroitin glucuronyltransferase 2; Chondroitin-polymerizing factor; ChPF; Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II; N-acetylgalactosaminyl-pro |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 79586 |
| Human Swiss-Prot | Q8IZ52 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CHSY2 |
| Fields | >>Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate;>>Metabolic pathways |
| Gene name | CHPF |
| Protein name | Chondroitin sulfate synthase 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 74241 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q6IQX7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CHSY2. AA range:631-680 |
| Specificity | CHSY2 Polyclonal Antibody detects endogenous levels of CHSY2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 85kD |
| Background | catalytic activity:UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.,catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.,cofactor:Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt.,function:Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.,online information:GlycoGene database,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the chondroitin N-acetylgalactosaminyltransferase family.,subunit:Binds CHSY1.,tissue specificity:Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, small intestine and placenta., |
| Function | catalytic activity:UDP-alpha-D-glucuronate + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan.,catalytic activity:UDP-N-acetyl-D-galactosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan = UDP + N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan.,cofactor:Divalent cations. Highest activities are measured with manganese. Can also utilize cobalt.,function:Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer.,online information:GlycoGene database,PTM:Phosphorylated upon DNA damage, p |
| Subcellular location | [Isoform 1]: Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein . Cytoplasm, cytosol .; [Isoform 3]: Cytoplasm, cytosol . Mitochondrion .; [Isoform 2]: Mitochondrion matrix . |
| Expression | Ubiquitous. Highly expressed in pancreas, ovary, brain, heart, skeletal muscle, colon, kidney, liver, stomach, spleen and placenta. ; [Isoform 2]: Expressed in brain, spleen, ovary, testis, lung and peripheral mononuclear cells. ; [Isoform 3]: Also ubiquitous. |
Additional Images
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Immunofluorescence analysis of MCF7 cells, using CHSY2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using CHSY2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO and K562 cells, using CHSY2 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES7583-100
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