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Antibody Product Overview
| ELK.No | ES7728 |
| Product name | USP44 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | USP44; Ubiquitin carboxyl-terminal hydrolase 44; Deubiquitinating enzyme 44; Ubiquitin thioesterase 44; Ubiquitin-specific-processing protease 44 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 84101 |
| Human Swiss-Prot | Q9H0E7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | USP44 |
| Fields | |
| Gene name | USP44 |
| Protein name | Ubiquitin carboxyl-terminal hydrolase 44 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8C2S0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human USP44. AA range:211-260 |
| Specificity | USP44 Polyclonal Antibody detects endogenous levels of USP44 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 81kD |
| Background | Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP44 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes (Quesada et al., 2004 [PubMed 14715245]).[supplied by OMIM, Mar 2008], |
| Function | catalytic activity:Ubiquitin C-terminal thioester + H(2)O = ubiquitin + a thiol.,similarity:Belongs to the peptidase C19 family.,similarity:Contains 1 UBP-type zinc finger.,tissue specificity:Expressed in testis., |
| Subcellular location | Nucleus . Peaks in interphase, with relatively low levels maintained throughout mitosis. . |
| Expression | Expressed in testis. Expressed at high levels in T-cell acute lymphoblastic leukemia. |
Additional Images
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Western Blot analysis of 293T cells using USP44 Polyclonal Antibody diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of mouse-kidney lysis using USP44 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from LOVO and NIH/3T3 cells, using USP44 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES7728-50
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