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NIFK (phospho Thr234) rabbit pAb
AO-06-ES7753-50
Antibody Product Overview
| ELK.No | ES7753 |
| Product name | NIFK (phospho Thr234) rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | IHC;IF;ELISA |
| Other name | MKI67IP; NIFK; NOPP34; MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 84365 |
| Human Swiss-Prot | Q9BYG3 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NIFK |
| Fields | |
| Gene name | MKI67IP |
| Protein name | MKI67 FHA domain-interacting nucleolar phosphoprotein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67949 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q91VE6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NIFK around the phosphorylation site of Thr234. AA range:200-249 |
| Specificity | Phospho-NIFK (T234) Polyclonal Antibody detects endogenous levels of NIFK protein only when phosphorylated at T234. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 34kD |
| Observed band (KD) | |
| Background | This gene encodes a protein that interacts with the forkhead-associated domain of the Ki-67 antigen. The encoded protein may bind RNA and may play a role in mitosis and cell cycle progression. Multiple pseudogenes exist on chromosomes 5, 10, 12, 15, and 19.[provided by RefSeq, Jan 2009], |
| Function | PTM:Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Localizes to mitotic chromosomes in conjunction with MKI67.,subunit:Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis., |
| Subcellular location | Nucleus, nucleolus. Chromosome. Localizes to mitotic chromosomes in conjunction with MKI67. |
| Expression | Brain,Cervix carcinoma,Epithelium,Lung,Plac |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using NIFK (Phospho-Thr234) Antibody
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Immunofluorescence analysis of HUVEC cells, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using NIFK (Phospho-Thr234) Antibody. The picture on the right is blocked with the phospho peptide.
: AO-06-ES7753-50
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