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Antibody Product Overview
| ELK.No | ES7767 |
| Product name | TEF-4 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | TEAD2; TEF4; Transcriptional enhancer factor TEF-4; TEA domain family member 2; TEAD-2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8463 |
| Human Swiss-Prot | Q15562 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TEF-4 |
| Fields | >>Hippo signaling pathway;>>Hippo signaling pathway - multiple species |
| Gene name | TEAD2 |
| Protein name | Transcriptional enhancer factor TEF-4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 21677 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P48301 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TEAD2. AA range:71-120 |
| Specificity | TEF-4 Polyclonal Antibody detects endogenous levels of TEF-4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 49kD |
| Observed band (KD) | |
| Background | function:Putative transcription factor that binds to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.,similarity:Contains 1 TEA DNA-binding domain., |
| Function | function:Putative transcription factor that binds to the SPH and GT-IIC "enhansons" (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.,similarity:Contains 1 TEA DNA-binding domain., |
| Subcellular location | Nucleus. |
| Expression | Brain,Eye,Skin,Testis, |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,TEF-4 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-actin Monoclonal Antibody(5B7)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of mouse-brain cells using TEF-4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HepG2 cells, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TEAD2 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES7767-50
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