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CaMKIα (phospho Thr177) rabbit pAb
AO-06-ES7812-50
Antibody Product Overview
| ELK.No | ES7812 |
| Product name | CaMKIα (phospho Thr177) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CAMK1; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8536 |
| Human Swiss-Prot | Q14012 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CaMKIα |
| Fields | >>Calcium signaling pathway;>>Oxytocin signaling pathway;>>Aldosterone synthesis and secretion;>>Glioma |
| Gene name | CAMK1 |
| Protein name | Calcium/calmodulin-dependent protein kinase type 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 52163 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q91YS8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 171503 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63450 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192 |
| Specificity | Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 41kD |
| Background | Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By si |
| Subcellular location | Cytoplasm . Nucleus . Predominantly cytoplasmic. . |
| Expression | Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK1-alpha (Phospho-Thr177) Antibody
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Immunofluorescence analysis of HeLa cells, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CaMK1-alpha (Phospho-Thr177) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' and Jurkat cells treated with insulin 0.01U/ml 15', using CaMK1-alpha (Phospho-Thr177) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7812-50
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