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Mnk1 (phospho Thr255) rabbit pAb
AO-06-ES7822-50
Antibody Product Overview
| ELK.No | ES7822 |
| Product name | Mnk1 (phospho Thr255) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | MKNK1; MNK1; MAP kinase-interacting serine/threonine-protein kinase 1; MAP kinase signal-integrating kinase 1; MAPK signal-integrating kinase 1; Mnk1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8569 |
| Human Swiss-Prot | Q9BUB5 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Mnk1 |
| Fields | >>MAPK signaling pathway;>>HIF-1 signaling pathway;>>Insulin signaling pathway |
| Gene name | MKNK1 |
| Protein name | MAP kinase-interacting serine/threonine-protein kinase 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17346 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O08605 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 500526 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q4G050 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MNK1 around the phosphorylation site of Thr255. AA range:221-270 |
| Specificity | Phospho-Mnk1 (T255) Polyclonal Antibody detects endogenous levels of Mnk1 protein only when phosphorylated at T255. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | MAP kinase interacting serine/threonine kinase 1(MKNK1) Homo sapiens This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Phosphorylated and activated by the p38 kinases and kinases in the Erk pathway.,function:May play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.,PTM:Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.,tissue specificity:Ubiquitous., |
| Subcellular location | [Isoform 2]: Cytoplasm.; [Isoform 3]: Cytoplasm. Nucleus. |
| Expression | Ubiquitous. |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MNK1 (Phospho-Thr255) Antibody
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Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ug/ml 24h, using MNK1 (Phospho-Thr255) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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