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Antibody Product Overview
| ELK.No | ES7848 |
| Product name | eIF3α rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | EIF3J; EIF3S1; Eukaryotic translation initiation factor 3 subunit J; eIF3j; Eukaryotic translation initiation factor 3 subunit 1; eIF-3-alpha; eIF3 p35 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8669 |
| Human Swiss-Prot | O75822 |
| Source | Rabbit |
| Isotype | IgG |
| Target | eIF3α |
| Fields | |
| Gene name | EIF3J |
| Protein name | Eukaryotic translation initiation factor 3 subunit J |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 691947 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | A0JPM9 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from eIF3α . at AA range: 40-120 |
| Specificity | eIF3α Polyclonal Antibody detects endogenous levels of eIF3α protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 30kD |
| Background | This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2013], |
| Function | function:Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.,mass spectrometry: PubMed:17322308,mass spectrometry: PubMed:18599441,P |
| Subcellular location | Cytoplasm . |
| Expression | Epithelium,Fetal liver,Lung carcinoma,Ovarian carci |
Additional Images
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Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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: AO-06-ES7848-50
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