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CD5 (phospho Tyr453) rabbit pAb
AO-06-ES7989-100
Antibody Product Overview
| ELK.No | ES7989 |
| Product name | CD5 (phospho Tyr453) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CD5; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD antigen CD5 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 921 |
| Human Swiss-Prot | P06127 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CD5 |
| Fields | >>Hematopoietic cell lineage |
| Gene name | CD5 |
| Protein name | T-cell surface glycoprotein CD5 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12507 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P13379 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 54236 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P51882 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CD5 around the phosphorylation site of Tyr453. AA range:421-470 |
| Specificity | Phospho-CD5 (Y453) Polyclonal Antibody detects endogenous levels of CD5 protein only when phosphorylated at Y453. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | function:May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.,similarity:Contains 3 SRCR domains., |
| Function | function:May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.,similarity:Contains 3 SRCR domains., |
| Subcellular location | Cell membrane; Single-pass type I membrane protein. |
| Expression | Lymphocyte,Pancreas,Tonsil, |
Additional Images
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Western Blot analysis of 293 cells using Phospho-CD5 (Y453) Polyclonal Antibody
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD5 (Phospho-Tyr453) Antibody
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Immunofluorescence analysis of HepG2 cells, using CD5 (Phospho-Tyr453) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from 293 cells treated with PMA 125ng/ml 30' , using CD5 (Phospho-Tyr453) Antibody. The lane on the right is blocked with the phospho peptide.
: AO-06-ES7989-100
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