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Antibody Product Overview
| ELK.No | ES8046 |
| Product name | CRSP34 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | MED27; CRSP34; CRSP8; Mediator of RNA polymerase II transcription subunit 27; Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional co |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9442 |
| Human Swiss-Prot | Q6P2C8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CRSP34 |
| Fields | >>Thyroid hormone signaling pathway |
| Gene name | MED27 |
| Protein name | Mediator of RNA polymerase II transcription subunit 27 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 68975 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9DB40 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from CRSP34 . at AA range: 90-170 |
| Specificity | CRSP34 Polyclonal Antibody detects endogenous levels of CRSP34 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 35kD |
| Background | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011], |
| Function | function:Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.,sequence caution:Translated as Gln.,sequence caution:Translation N-terminally shortened.,similarity:Belongs to the Mediator complex subunit 27 family.,subunit:Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, ME |
| Subcellular location | Nucleus . |
| Expression | Lung,Skin, |
Additional Images
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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
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: AO-06-ES8046-100
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