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Antibody Product Overview
| ELK.No | ES8072 |
| Product name | ARA54 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | RNF14; ARA54; HRIHFB2038; E3 ubiquitin-protein ligase RNF14; Androgen receptor-associated protein 54; HFB30; RING finger protein 14; Triad2 protein |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9604 |
| Human Swiss-Prot | Q9UBS8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ARA54 |
| Fields | |
| Gene name | RNF14 |
| Protein name | E3 ubiquitin-protein ligase RNF14 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 56736 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9JI90 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF14. AA range:361-410 |
| Specificity | ARA54 Polyclonal Antibody detects endogenous levels of ARA54 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 50kD |
| Background | The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Six alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq, Jan 2011], |
| Function | caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,domain:The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.,domain:The RING-type zinc finger is essential for the interaction with UBE2E2.,function:Might act as an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates, which could be nuclear proteins. Could play a role as a coactivator for androgen- and, to a lesser extent, progesterone-dependent transcription.,pathway:Protein modification; protein ubiquitination.,PTM:RING-type zinc finger-dependent and UBE2E2-dependent autoubiquitination.,similarity:Be |
| Subcellular location | Cytoplasm . Nucleus . |
| Expression | Widely expressed. |
Additional Images
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Western blot analysis of lysates from RAW264.7 cells, using RNF14 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES8072-100
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