Français
English
Antibody Product Overview
| ELK.No | ES8073 |
| Product name | RIN1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | RIN1; Ras and Rab interactor 1; Ras inhibitor JC99; Ras interaction/interference protein 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9610 |
| Human Swiss-Prot | Q13671 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RIN1 |
| Fields | >>Ras signaling pathway |
| Gene name | RIN1 |
| Protein name | Ras and Rab interactor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 225870 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q921Q7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 207119 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P97680 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RIN1. AA range:655-704 |
| Specificity | RIN1 Polyclonal Antibody detects endogenous levels of RIN1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 84kD |
| Background | function:Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.,PTM:Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylated on Ser-351 by PKD.,similarity:Belongs to the RIN (Ras interaction/interference) family.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 VPS9 domain.,subcellular location:Some amount is membrane-associated.,subunit:Interacts with the GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). This interaction prevents the association between RAF1 and Ras. Interacts with 14-3-3 proteins YWHAB, YWHAE and YWHAZ when phosphorylated on Ser-351. Interacts with the SH3 domain of ABL1 and ABL2. Interacts with RAB5A. The interaction with Ras is probably regulated and antagonized by the interaction with 14-3-3 proteins. The interaction with 14-3-3 proteins is regulated by phosphorylation on Ser-351.,tissue specificity:Expressed in all tissues examined with high levels in brain, placenta and pancreas., |
| Function | function:Ras effector protein, which may serve as an inhibitory modulator of neuronal plasticity in aversive memory formation. Can affect Ras signaling at different levels. First, by competing with RAF1 protein for binding to activated Ras. Second, by enhancing signaling from ABL1 and ABL2, which regulate cytoskeletal remodeling. Third, by activating RAB5A, possibly by functioning as a guanine nucleotide exchange factor (GEF) for RAB5A, by exchanging bound GDP for free GTP, and facilitating Ras-activated receptor endocytosis.,PTM:Phosphorylated on tyrosine residues by ABL1 and ABL2. Phosphorylated on Ser-351 by PKD.,similarity:Belongs to the RIN (Ras interaction/interference) family.,similarity:Contains 1 Ras-associating domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 VPS9 domain.,subcellular location:Some amount is membrane-associated.,subunit:Interacts with the GTP-boun |
| Subcellular location | Cytoplasm . Membrane . Cytoplasm, cytoskeleton . Some amount is membrane-associated. |
| Expression | Expressed in all tissues examined with high levels in brain, placenta and pancreas. |
Additional Images
No image
Western blot analysis of lysates from K562 cells, using RIN1 Antibody. The lane on the right is blocked with the synthesized peptide.
No image
Western blot analysis of the lysates from COLO205 cells using RIN1 antibody.
No image
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
No image
: AO-06-ES8073-50
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package