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Antibody Product Overview
| ELK.No | ES8099 |
| Product name | Repac rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC |
| Other name | RAPGEF5; GFR; KIAA0277; MRGEF; Rap guanine nucleotide exchange factor 5; Guanine nucleotide exchange factor for Rap1; M-Ras-regulated Rap GEF; MR-GEF; Related to Epac; Repac |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9771 |
| Human Swiss-Prot | Q92565 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Repac |
| Fields | >>Ras signaling pathway;>>Rap1 signaling pathway |
| Gene name | RAPGEF5 |
| Protein name | Rap guanine nucleotide exchange factor 5 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 217944 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8C0Q9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 362799 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P83900 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RAPGEF5. AA range:431-480 |
| Specificity | Repac Polyclonal Antibody detects endogenous levels of Repac protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008], |
| Function | function:Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation.,similarity:Contains 1 N-terminal Ras-GEF domain.,similarity:Contains 1 Ras-GEF domain.,tissue specificity:Widely expressed with highest levels in brain., |
| Subcellular location | Nucleus. |
| Expression | Widely expressed with highest levels in brain. |
Additional Images
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Western blot analysis of lysates from RAW264.7 cells, using RAPGEF5 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using RAPGEF5 antibody.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES8099-100
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