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Antibody Product Overview
| ELK.No | ES8351 |
| Product name | Topo IIIα rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | TOP3A; TOP3; DNA topoisomerase 3-alpha; DNA topoisomerase III alpha |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 7156 |
| Human Swiss-Prot | Q13472 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Topo IIIα |
| Fields | >>Homologous recombination;>>Fanconi anemia pathway |
| Gene name | TOP3A |
| Protein name | DNA topoisomerase 3-alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 21975 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O70157 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from Topo IIIα . at AA range: 350-430 |
| Specificity | Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 115kD |
| Background | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016], |
| Function | catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,function:Reduces the number of supercoils in a highly negatively supercoiled DNA. Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells.,similarity:Belongs to the prokaryotic type I/III topoisomerase family.,subunit:Directly interacts with BLM and RMI1. Component of the RMI complex, containing at least TOP3A, RMI1 and RMI2. The RMI complex interacts with BLM.,tissue specificity:High expression is found in testis, heart, skeletal muscle and pancreas., |
| Subcellular location | Mitochondrion matrix . |
| Expression | High expression is found in testis, heart, skeletal muscle and pancreas. |
Additional Images
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Western blot analysis of 293T using Topo IIIα antibody. Antibody was diluted at 1:500. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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: AO-06-ES8351-100
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