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Antibody Product Overview
| ELK.No | ES8354 |
| Product name | MRP-L35 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC |
| Other name | MRPL35; BM-007; 39S ribosomal protein L35, mitochondrial; L35mt; MRP-L35 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 51318 |
| Human Swiss-Prot | Q9NZE8 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-L35 |
| Fields | >>Ribosome |
| Gene name | MRPL35 |
| Protein name | 39S ribosomal protein L35 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 66223 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9CQL6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from MRP-L35 . at AA range: 80-160 |
| Specificity | MRP-L35 Polyclonal Antibody detects endogenous levels of MRP-L35 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 21kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008], |
| Function | similarity:Belongs to the ribosomal protein L35P family., |
| Subcellular location | Mitochondrion . |
| Expression | Bone marrow,Liver,Stomach, |
Additional Images
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Western blot analysis of 293T COLO using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western blot analysis of 293 using MRP-L35 antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES8354-50
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