Anti-UHRF1 mAb

Recombinant Anti-IKB alpha antibody (Mouse mAb), Primary Antibody

AO-03-GB151509-50

Recombinant Anti-IKB alpha antibody (Mouse mAb)

Volume : 50 µL
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Recombinant Anti-IKB alpha antibody (Mouse mAb)

Protein full name

NF-kappa-B inhibitor alpha

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA

Immunogen

KLH conjugated Synthetic peptide corresponding to Human IKBα

Uniprot ID

P25963

Isotype

IgG1

Purity

Affinity purification

Predicted MW. / Observed MW.

36 kDa / 36 kDa

Applications

WB

Human, Mouse, Rat

1: 1000-1: 2000

HeLa+Calyculin A,NIH/3T3+Calyculin A, HeLa, NIH3T3

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Images

01 GB151509_IKB_重组M00323072702_d1000_WB.png

WB analysis of IKB alpha (GB151509).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1:1000, 4℃ overnight.

Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

Storage

Storage             Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB151509-50
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Hurry! only 10 items left in stock.

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