Anti-UHRF1 mAb

Recombinant Anti-Phospho-AKT(Ser473) antibody (Rabbit mAb), Primary Antibody

AO-03-GB150002-50

Recombinant Anti-Phospho-AKT(Ser473) antibody (Rabbit mAb)

Volume : 50 µL
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Recombinant Anti-Phospho-AKT(Ser473) antibody

Protein full name

RAC-beta serine/threonine-protein kinase， RAC-gamma serine/threonine-protein kinase， RAC-alpha serine/threonine-protein kinase

Synonyms

AKT, AKT (pan), AKT pan, AKT1, AKT1/2/3, pan AKT, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha, total AKT

Immunogen

KLH conjugated Synthetic phosphopeptide corresponding to Human AKT(Ser473)

Uniprot ID

P31751, Q9Y243, P31749

Isotype

IgG

Purity

Affinity purification

Predicted MW. / Observed MW.

56 kDa / 60 kDa

Applications

WB

Human, Mouse, Rat

1: 1000-1: 2000

HeLa,NIH3T3,C6

Background

An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK.These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr.Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation.While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery.

Images

01 GB150002_Phospho-AKT(Ser473)_重组H299_d8000_WB_WM.png

WB analysis of Phospho-AKT(Ser473)(GB150002).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 2000, 4℃ overnight.

Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

Storage

Storage             Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB150002-50
: 10 Produits
Hurry! only 10 items left in stock.

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