Anti-UHRF1 mAb

Anti-UCP1 Rabbit pAb, Primary Antibody

AO-03-GB112174-50

Anti-UCP1 Rabbit pAb

Volume : 50 µL
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Anti-UCP1 Rabbit pAb

Description

UCP1 rabbit polyclonal antibody

Protein full name

Mitochondrial brown fat uncoupling protein 1

Synonyms

UCP1, Solute carrier family 25 member 7, Thermogenin, Slc25a7

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse UCP1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Mitochondrion

Predicted MW.

33 kDa

Observed MW.

33 kDa

Uniprot ID

P12242, P04633

Applications

WB

Mouse, Rat

1: 500-1: 1000

brown fat

IHC/IF

Mouse, Rat

1: 200-1: 600

brown adipose tissue

Background

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

Images

1639989809030444762.png

WB analysis of UCP1 (GB112174).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

1639989815533509588.png

IHC analysis of UCP1 (GB112174).
Sample: Mouse brown fat (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

1639989815535801993.png

IHC analysis of UCP1 (GB112174).
Sample: Rat brown fat (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 300, 4℃ overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h.

Storage

Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

: AO-03-GB112174-50
: 10 Produits
Hurry! only 10 items left in stock.

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