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Anti-Osteopontin Rabbit pAb, Primary Antibody
AO-03-GB11500-50
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Anti-Osteopontin Rabbit pAb
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Protein full name |
Osteopontin |
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Synonyms |
SPP1, BNSP, BSPI, ETA-1, OPN, secreted phosphoprotein 1, Osteopontin |
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Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse Osteopontin |
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Uniprot ID |
P10923 |
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Isotype |
IgG |
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Purity |
Affinity purification |
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Subcellular location |
Secreted |
Applications
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 2000 |
bone |
Background
Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by theSPP1 gene (secreted phosphoprotein 1).Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite.
Images
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IHC analysis of OPN (GB11500). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of OPN (GB11500). Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of OPN (GB11500). Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of OPN (GB11500). Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98℃,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4℃ overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IHC analysis of OPN(GB11500).Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201)，98℃，20 min. Primary antibody:1: 100, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
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IHC analysis of OPN(GB11500). Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201)，98℃，20 min. Primary antibody:1: 500, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
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IHC analysis of OPN(GB11500). Sample: Human bone,4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201)，98℃，20 min. Primary antibody:1: 500, 4℃ overnight. Secondary antibody:HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.
: AO-03-GB11500-50
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